BSA Mouse Monoclonal Antibody [A7D12]
cat.: HA600087
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Cow
Applications: ELISA
Clonality: Monoclonal
Clone number: A7D12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 66 kDa
Isotype: IgG1
Immunogen: Native protein.
Positive control: BSA protein.
Subcellular location: Secreted.
Recommended Dilutions:
  ELISA

1:10,000
Uniprot #: SwissProt: P02769 Bovine
Alternative names: Albumin BSA Bos d 6 ALB
Images
HA600087_1.jpg Fig1: Bovine Serum Albumin Antibody (HA600087) in indirect ELISA.

Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA600087_2.jpg Fig2: Bovine Serum Albumin Antibody (HA600087) in ELISA.

Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 0.8 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

This antibody demonstrates high specificity for BSA and little or no crossreactivity to HSA (Human serum albumin), Goat serum and Rabbit serum.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.