Galactosidase alpha Mouse Monoclonal Antibody [A7C4]
cat.: HA600088
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A7C4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human Galactosidase alpha aa 51-300/429. |
| Positive control: | MCF-7 cell lysates, human adrenal gland tissue, human kidney tissue. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:8,000 1:600 |
| Uniprot #: | SwissProt: P06280 Human |
| Alternative names: | AGAL_HUMAN Agalsidase alfa Alpha D galactosidase A Alpha D galactoside galactohydrolase 1 Alpha D galactoside galactohydrolase Alpha gal A Alpha galactosidase A Alpha-D-galactosidase A Alpha-D-galactoside galactohydrolase Alpha-galactosidase A GALA Galactosidase, alpha GLA GLA protein Melibiase |
Images
|
Fig1:
Western blot analysis of Galactosidase alpha on MCF-7 cell lysates with Mouse anti-Galactosidase alpha antibody (HA600088) at 1/8,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 49 kDa Observed band size: 49/35 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600088) at 1/8,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Mouse anti-Galactosidase alpha antibody (HA600088) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600088) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Galactosidase alpha antibody (HA600088) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600088) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.