Sall4 Mouse Monoclonal Antibody [A7A6]
cat.: HA600089
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A7A6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Sall4 aa 903-1,053/1,053 |
| Positive control: | NCCIT cell lysates, NCCIT, human seminoma tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:100 1:600 1:500-1:10,000 |
| Uniprot #: | SwissProt: Q9UJQ4 Human |
| Alternative names: | AA407717 AL022809 AW536104 C330011P20Rik C78083 C78563 dJ1112F19.1 DRRS HSAL4 Sal like 4 (Drosophila) Sal like 4 Sal like Protein 4 Sal-like protein 4 Sall4 SALL4_HUMAN Spalt like transcription factor 4 Tex20 Zinc finger protein 797 Zinc finger protein SALL4 ZNF797 |
Images
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Fig1:
Western blot analysis of Sall4 on NCCIT cell lysates with Mouse anti-Sall4 antibody (HA600089) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 112 kDa Observed band size: 140/110/75 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600089) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NCCIT cells labeling Sall4 with Mouse anti-Sall4 antibody (HA600089) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Sall4 antibody (HA600089) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-Sall4 antibody (HA600089) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600089) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of NCCIT cells labeling Sall4. Cells were fixed and permeabilized, and then blocked with 2% negative goat serum for 15 minutes at room temperature.Then stained with the primary antibody (HA600089, 0.1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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