Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B5]
cat.: HA600090
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, ChIP
Clonality: Monoclonal
Clone number: A7B5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 15 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human Histone H3 aa 1-50 / 136.
Positive control: Hela treated with TSA cell lysate, rat liver tissue, rat hippocampus tissue, rat kidney tissue, rat myocardium tissue, human lung tissue, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human spleen tissue, human breast carcinoma tissue, human esophagus tissue, human placenta tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P
  ChIP
1:2,000
1:500
Use 0.5~2 μg for 25 μg of chromatin.
Uniprot #: SwissProt: P68431 Human | P84243 Human | Q16695 Human | Q6NXT2 Human | Q71DI3 Human | Q6LED0 Rat
Alternative names: H3.3A HIST1 cluster, H3E H3 histone family, member A H3.1 H3/l H3F3 H3FF H3FJ H3FL Histone gene cluster 1, H3 histone family, member E histone H3.1t Histone H3/o FLJ92264 H 3 H3 H3 histone family, member B H3 histone family, member C H3 histone family, member D H3 histone family, member F H3 histone family, member H H3 histone family, member I H3 histone family, member J H3 histone family, member K H3 histone family, member L H3 histone family, member T H3 histone, family 3A H3/A H3/b H3/c H3/d h3/f H3/h H3/i H3/j H3/k H3/t H31_HUMAN H3F1K H3F3A H3FA H3FB H3FC H3FD H3FH H3FI H3FK HIST1 cluster, H3A HIST1 cluster, H3B HIST1 cluster, H3C HIST1 cluster, H3D HIST1 cluster, H3F HIST1 cluster, H3G HIST1 cluster, H3H HIST1 cluster, H3I HIST1 cluster, H3J HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST3H3 Histone 1, H......
Images
HA600090_1.jpg Fig1: Western blot analysis of Histone H3 (acetyl K18) on different lysates with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/2,000 dilution.

Lane 1: Hela cell lysate
Lane 2: Hela treated with TSA cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 14/40 kDa

Exposure time: 30 seconds;
15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA600090_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat myocardium tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_13.jpg Fig13: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600090_14.jpg Fig14: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (acetyl K18) (HA600090) or Normal Mouse IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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