Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]
cat.: HA600091
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A7B6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Histone H3 aa 1-50 / 136. |
| Positive control: | Hela treated with TSA cell lysate, Hela, TSA treated Hela. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P68431 Human | P84243 Human | Q16695 Human | Q6NXT2 Human | Q71DI3 Human |
| Alternative names: | H3.3A HIST1 cluster, H3E H3 histone family, member A H3.1 H3/l H3F3 H3FF H3FJ H3FL Histone gene cluster 1, H3 histone family, member E histone H3.1t Histone H3/o FLJ92264 H 3 H3 H3 histone family, member B H3 histone family, member C H3 histone family, member D H3 histone family, member F H3 histone family, member H H3 histone family, member I H3 histone family, member J H3 histone family, member K H3 histone family, member L H3 histone family, member T H3 histone, family 3A H3/A H3/b H3/c H3/d h3/f H3/h H3/i H3/j H3/k H3/t H31_HUMAN H3F1K H3F3A H3FA H3FB H3FC H3FD H3FH H3FI H3FK HIST1 cluster, H3A HIST1 cluster, H3B HIST1 cluster, H3C HIST1 cluster, H3D HIST1 cluster, H3F HIST1 cluster, H3G HIST1 cluster, H3H HIST1 cluster, H3I HIST1 cluster, H3J HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST3H3 Histone 1, H...... |
Images
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Fig1:
Western blot analysis of Histone H3 (acetyl K18) on different lysates with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/2,000 dilution. Lane 1: Hela cell lysate Lane 2: Hela treated with TSA cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 14 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of TSA treated Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.