Lamin A + Lamin C Mouse Monoclonal Antibody [A6F5]
cat.: HA600093
| Product Type: | Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A6F5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 74/65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Lamin A/C aa 151-350/664. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysates, human skin tissue, rat skeletal muscle tissue, rat kidney tissue, rat heart tissue. |
| Subcellular location: | Nucleus, Nucleus envelope, Nucleus lamina, Nucleoplasm, Nucleus matrix; Nucleus speckle. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:600 |
| Uniprot #: | SwissProt: P02545 Human | P48678 Mouse | P48679 Rat |
| Alternative names: | 70 kDa lamin CDDC EMD2 FPL FPLD HGPS IDC LAMIN A lamin A/C LAMIN C Lamin-A/C LDP1 LFP LMN 1 LMN A LMN C LMNA LMNA_HUMAN LMNC PRO1 Renal carcinoma antigen NY-REN-32 |
Images
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Fig1:
Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600093) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Lamin A + Lamin C on PC-12 cell lysates with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 4 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600093) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600093) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600093) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600093) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600093) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.