Phospho-mTOR (S2448) Mouse Monoclonal Antibody [A5D5]
cat.: HA600094
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A5D5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 289 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human mTOR aa 2430-2480. |
| Positive control: | HeLa cell lysate, HeLa starved overnight then treated with 200nM PMA for 4 hours cell lysate, NIH/3T3 cell lysate treated with serum-starvation overnight, NIH/3T3 cell lysate treated with Insulin (100nM) for 10 minutes after serum-starvation overnight, human liver tissue. |
| Subcellular location: | Lysosome, Mitochondrion outer membrane, Endoplasmic reticulum membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P42345 Human | Q9JLN9 Mouse |
| Alternative names: | dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) FK506 binding protein 12 rapamycin associated protein 1 FK506 binding protein 12 rapamycin associated protein 2 FK506 binding protein 12 rapamycin complex associated protein 1 FK506-binding protein 12-rapamycin complex-associated protein 1 FKBP rapamycin associated protein FKBP12 rapamycin complex associated protein FKBP12-rapamycin complex-associated protein 1 FKBP12-rapamycin complex-associated protein FLJ44809 FRAP FRAP1 FRAP2 Mammalian target of rapamycin Mechanistic target of rapamycin mTOR MTOR_HUMAN OTTHUMP00000001983 RAFT1 Rapamycin and FKBP12 target 1 Rapamycin associated protein FRAP2 Rapamycin target protein 1 Rapamycin target protein RAPT1 Serine/threonine-protein kinase mTOR |
Images
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Fig1:
Western blot analysis of Phospho-mTOR (S2448) on different lysates with Mouse anti-Phospho-mTOR (S2448) antibody (HA600094) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 289 kDa Observed band size: 289 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600094) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Phospho-mTOR (S2448) on different lysates with Mouse anti-Phospho-mTOR (S2448) antibody (HA600094) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate treated with serum-starvation overnight Lane 2: NIH/3T3 cell lysate treated with Insulin (100nM) for 10 minutes after serum-starvation overnight Lysates/proteins at 10 µg/Lane. Predicted band size: 288 kDa Observed band size: 255 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA for 1 hour at room temperature. The primary antibody (HA600094) at 1/500 dilution was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:40,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Phospho-mTOR (S2448) antibody (HA600094) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600094) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.