BCL6 Mouse Monoclonal Antibody [A6C7]
cat.: HA600096
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: A6C7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 79 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human BCL-6 aa 301-500 / 706.
Positive control: Human lymph nodes tissue, human B lymphocytoma tissue, Raji.
Subcellular location: Nucleus.
Recommended Dilutions:
  IHC-P
  IF-Cell

1:500
1:200
Uniprot #: SwissProt: P41182 Human
Alternative names: B cell CLL/lymphoma 6 B cell lymphoma 6 protein B-cell lymphoma 5 protein B-cell lymphoma 6 protein BCL 5 Bcl 6 BCL-5 BCL-6 BCL5 BCL6 BCL6_HUMAN BCL6A cys his2 zinc finger transcription factor cys-his2 zinc finger transcription factor LAZ 3 LAZ 3 protein LAZ3 Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) Lymphoma associated zinc finger gene on chromosome 3 Protein LAZ-3 ZBTB 27 ZBTB27 Zinc finger and BTB domain containing protein 27 Zinc finger and BTB domain-containing protein 27 (ZBTB27) Zinc finger and BTB domain-containing protein 27 Zinc Finger Protein 51 (ZNF51) Zinc finger protein 51 zinc finger transcription factor BCL6S ZNF 51 ZNF51
Images
HA600096_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-BCL6 antibody (HA600096) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600096) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600096_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-BCL6 antibody (HA600096) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600096) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600096_3.jpg Fig3: Immunocytochemistry analysis of Raji cells labeling BCL6 with Mouse anti-BCL6 antibody (HA600096) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-BCL6 antibody (HA600096) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.