xCT/SLC7A11 Mouse Monoclonal Antibody [A7C5]
cat.: HA600097
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A7C5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG2b
Immunogen: 293 cell line overexpressing SLC7A11.
Positive control: HT-29 cell lysate, A549 cell lysate, human pancreas tissue, A549, HT-29.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:200
1:50-1:100
Uniprot #: SwissProt: Q9UPY5 Human | Q9WTR6 Mouse
Alternative names: Amino acid transport system xc xCT Amino acid transport system xc- Calcium channel blocker resistance protein CCBR1 Calcium channel blocker resistance protein CCBR1 CCBR1 Cysteine/glutamate transporter Cystine/glutamate transporter OTTHUMP00000164578 SLC7A11 Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 solute carrier family 7 Solute carrier family 7 member 11 Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN xCT XCT_HUMAN
Images
HA600097_1.jpg Fig1: Western blot analysis of xCT/SLC7A11 on different lysates with Mouse anti-xCT/SLC7A11 antibody (HA600097) at 1/500 dilution.

Lane 1: HT-29 cell lysate
Lane 2: A549 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 50 kDa

Exposure time: 30 seconds;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600097) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA600097_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT/SLC7A11 antibody (HA600097) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600097) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600097_3.jpg Fig3: Flow cytometric analysis of A549 cells labeling xCT/SLC7A11.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA600097, 10ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA600097_4.jpg Fig4: Flow cytometric analysis of HT-29 cells labeling xCT/SLC7A11.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA600097, 10ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.