Prolactin Receptor Mouse Monoclonal Antibody [A6E4]
cat.: HA600099
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A6E4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Prolactin Receptor aa 150-350 / 622. |
| Positive control: | 293T cell lysate, Hela cell lysate, MCF-7, human kidney tissue, human pancreas tissue, HeLa. |
| Subcellular location: | Membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100 1:200 1:1,000 |
| Uniprot #: | SwissProt: P16471 Human |
| Alternative names: | AI987712 CLONE SPM213 CPRLP Delta 4-delta 7/11 truncated prolactin receptor Delta 4-SF1b truncated prolactin receptor HPRL hPRL receptor hPRLrI Lactogen receptor MFAB MGC105486 OPR OTTHUMP00000115998 Pr-1 Pr-3 PRL R PRL-R PRLR Prlr-rs1 PRLR_HUMAN Prolactin receptor a Prolactin receptor Prolactin receptor delta 7/11 RATPRLR Secreted prolactin binding protein Truncated testis-specific box 1-C prolactin receptor wu:fj65c07 |
Images
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Fig1:
Western blot analysis of Prolactin Receptor on different lysates with Mouse anti-Prolactin Receptor antibody (HA600099) at 1/500 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600099) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling Prolactin Receptor with Mouse anti-Prolactin Receptor antibody (HA600099) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Prolactin Receptor antibody (HA600099) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Prolactin Receptor antibody (HA600099) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600099) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Prolactin Receptor antibody (HA600099) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600099) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of HeLa cells labeling Prolactin Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (HA600099, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.