Prolactin Receptor / PRL-R Mouse Monoclonal Antibody [A6E4]
cat.: HA600099
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A6E4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Prolactin Receptor aa 150-350 / 622. |
| Positive control: | MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, MCF-7, human kidney tissue, human pancreas tissue, HeLa. |
| Subcellular location: | Membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:100 1:200 1:1,000 |
| Uniprot #: | SwissProt: P16471 Human |
| Alternative names: | AI987712 CLONE SPM213 CPRLP Delta 4-delta 7/11 truncated prolactin receptor Delta 4-SF1b truncated prolactin receptor HPRL hPRL receptor hPRLrI Lactogen receptor MFAB MGC105486 OPR OTTHUMP00000115998 Pr-1 Pr-3 PRL R PRL-R PRLR Prlr-rs1 PRLR_HUMAN Prolactin receptor a Prolactin receptor Prolactin receptor delta 7/11 RATPRLR Secreted prolactin binding protein Truncated testis-specific box 1-C prolactin receptor wu:fj65c07 |
Images
|
Fig1:
Western blot analysis of Prolactin Receptor / PRL-R on different lysates with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/2,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA600099, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 70 kDa Observed band size: 90 kDa |
|
Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling Prolactin Receptor / PRL-R with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600099) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Prolactin Receptor / PRL-R antibody (HA600099) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600099) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Flow cytometric analysis of HeLa cells labeling Prolactin Receptor / PRL-R. Cells were fixed and permeabilized. Then stained with the primary antibody (HA600099, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.