Integrin alpha 3 Mouse Monoclonal Antibody [A7H2]
cat.: HA600100
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A7H2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human Integrin alpha-3 aa 51-250 / 1,051. |
| Positive control: | HeLa cell lysate, HUVEC cell lysate, A431 cell lysate, human kidney tissue lysate, U-87 MG. |
| Subcellular location: | Cell membrane, invadopodium membrane, filopodium membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:100 |
| Uniprot #: | SwissProt: P26006 Human |
| Alternative names: | AA407068 Antigen identified by monoclonal J143 CD49 antigen like family member C CD49 antigen-like family member C CD49c CD49c antigen FLJ34631 FLJ34704 FRP 2 FRP-2 FRP2 Galactoprotein B3 GAP B3 GAPB3 Integrin alpha 3 Integrin alpha-3 light chain Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) ITA3_HUMAN ITGA 3 Itga3 MSK18 VCA 2 VCA2 Very late activation protein 3 receptor alpha 3 subunit VL3A VLA 3 alpha chain VLA 3 subunit alpha VLA-3 subunit alpha VLA3a |
Images
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Fig1:
Western blot analysis of Integrin alpha 3 on different lysates with Mouse anti-Integrin alpha 3 antibody (HA600100) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HUVEC cell lysate (10 µg/Lane) Lane 3: A431 cell lysate (10 µg/Lane) Lane 4: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of U-87 MG cells labeling Integrin alpha 3 with Mouse anti-Integrin alpha 3 antibody (HA600100) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Integrin alpha 3 antibody (HA600100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.