iFluor™ 488 Conjugated GFAP Mouse Monoclonal Antibody [1-D4]
cat.: HA600101F
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | 1-D4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human GFAP. |
| Positive control: | Rat brain tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Tissue IHC-Fr |
1:100-1:200 1:200 |
| Uniprot #: | SwissProt: P14136 Human | P47819 Rat |
| Alternative names: | wu:fb34h11 ALXDRD cb345 etID36982.3 FLJ42474 FLJ45472 GFAP GFAP_HUMAN gfapl Glial fibrillary acidic protein Intermediate filament protein wu:fk42c12 xx:af506734 zgc:110485 |
Images
|
Fig1:
Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling GFAP with Mouse anti-GFAP antibody (HA600101F) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then incubated overnight with GFAP-iFluor™ 488 (HA600101F, green) at 1/100 dilution overnight at +4℃ , washed with PBS. Nuclear DNA was labelled in blue with DAPI. |
|
Fig2:
Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.