iFluor™ 647 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PD00-15]
cat.: HA600105F
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | PD00-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide. |
| Positive control: | Human breast tissue, MCF-7. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Tissue FC |
1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q01546 Human | Q7Z794 Human |
| Alternative names: | pan ck pan-ck panck |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling pan Cytokeratin (HA600105F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody pan Cytokeratin (HA600105F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig2:
Flow cytometric analysis of MCF-7 cells labeling pan Cytokeratin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with pan Cytokeratin (HA600105F, iFluor™ 647, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.