iFluor™ 594 Conjugated beta Tubulin Recombinant Mouse Monoclonal Antibody [A1-A4-R]
cat.: HA600110F
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | A1-A4-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Beta tubulin aa 151-200 / 444. |
| Positive control: | HeLa, NIH/3T3, human kidney tissue, mouse testis tissue, rat kidney tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
IF-Cell IF-Tissue FC |
1:100 1:200 1:1,000 |
| Uniprot #: | SwissProt: P07437 Human | P99024 Mouse | P69897 Rat |
| Alternative names: | beta 3 tubulin beta-4 CDCBM CDCBM1 CFEOM3 CFEOM3A FEOM3 M(beta)3 M(beta)6 MC1R Neuron specific beta III Tubulin Neuron-specific class III beta-tubulin QccE-11995 QccE-15186 TBB3_HUMAN Tubb 3 TUBB3 TUBB4 Tubulin beta 3 Tubulin beta 3 chain Tubulin beta 4 Tubulin beta III Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III |
Images
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Fig1:
Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling beta Tubulin (HA600110F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling beta Tubulin (HA600110F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded rat kidney tissue labeling beta Tubulin (HA600110F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig6:
Flow cytometric analysis of HeLa cells labeling beta Tubulin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with beta Tubulin (HA600110F, red, 1μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig7:
Flow cytometric analysis of NIH/3T3 cells labeling beta Tubulin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with beta Tubulin (HA600110F, red, 1μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.