iFluor™ 488 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PDH0-10]
cat.: HA600111F
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PDH0-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Full length native protein corresponding to Human pan Cytokeratin. |
| Positive control: | Human small intestine tissue, human skin tissue. |
| Subcellular location: | Cytoplasmic |
| Recommended Dilutions:
IF-Tissue |
1:200 |
| Uniprot #: | SwissProt: P13645 Human | P02533 Human | P19012 Human | P08779 Human | P08727 Human |
| Alternative names: | pan Cytokeratin pan-Cytokeratin pan ck pan-ck panck pan Keratin |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600111F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600111F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600111F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600111F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600111F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600111F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.