FITC Conjugated Myc tag Recombinant Mouse Monoclonal Antibody [A3C8-R]
cat.: HA600136F
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A3C8-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin. |
| Recommended Dilutions:
IF-Cell |
1:1,000 |
| Alternative names: | c-myc tag Myc Epitope Tag |
Images
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Fig1:
Immunocytochemistry analysis of 293T cells labeling Myc tag with Mouse anti-Myc tag antibody (HA600136F) at 1/1,000 dilution. 293T cells, transfected with empty control (top, negative) / Myc-tagged Histone H3.1 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myc tag antibody (HA600136F, FITC, green) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Histone H3 (ET1701-64, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.