FITC Conjugated DYKDDDDK Tag (FLAG) Recombinant Mouse Monoclonal Antibody [A2-A4-R]
cat.: HA600139F
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A2-A4-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide immune sequence is N-DYKDDDDK-C. |
| Recommended Dilutions:
IF-Cell |
1:500 |
| Alternative names: | DDDDK epitope tag DDDK ddk DYKDDDDK DYKDDDDK epitope tag DYKDDDDK tag ECS epitope tag ECS tag Enterokinase Cleavage Site epitope tag Enterokinase Cleavage Site tag FLAG FLAG tag |
Images
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Fig1:
Immunocytochemistry analysis of HeLa cells labeling DYKDDDDK Tag (FLAG) with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA600139F) at 1/500 dilution. HeLa cells, transfected with Flag-tagged Claudin 18.2 (top) / Flag-tagged Histone H3.1 (bottom) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA600139F, FITC, green) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Counterstained with Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.