Product Type: | Mouse monoclonal IgG1, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | A7F3 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 91 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within Human PDE4D aa 610-809 / 809. |
Positive control: | Hela cell lysates. |
Subcellular location: | Apical cell membrane, Cytoplasm, Cytoskeleton, Centrosome, Membrane. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: Q08499 Human |
Alternative names: | cAMP specific 3',5' cyclic phosphodiesterase 4D DKFZp686M11213 5''-cyclic phosphodiesterase 4D cAMP specific phosphodiesterase 4D cAMP specific phosphodiesterase PDE4D6 cAMP-specific 3'' DPDE3 Dunce like phosphodiesterase E3 FLJ97311 HSPDE4D PDE43 Pde4d PDE4D_HUMAN PDE4DN2 Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) Phosphodiesterase 4D cAMP specific Phosphodiesterase 4D, cAMP specific (dunce) STRK1 |
Fig1:
Western blot analysis of PDE4D on Hela cell lysates with Mouse anti-PDE4D antibody (HA601001) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601001) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |