PDE4D Mouse Monoclonal Antibody [A7F3]
cat.: HA601001
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A7F3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 91 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human PDE4D aa 610-809 / 809.
Positive control: Hela cell lysates.
Subcellular location: Apical cell membrane, Cytoplasm, Cytoskeleton, Centrosome, Membrane.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q08499 Human
Alternative names: cAMP specific 3',5' cyclic phosphodiesterase 4D DKFZp686M11213 5''-cyclic phosphodiesterase 4D cAMP specific phosphodiesterase 4D cAMP specific phosphodiesterase PDE4D6 cAMP-specific 3'' DPDE3 Dunce like phosphodiesterase E3 FLJ97311 HSPDE4D PDE43 Pde4d PDE4D_HUMAN PDE4DN2 Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) Phosphodiesterase 4D cAMP specific Phosphodiesterase 4D, cAMP specific (dunce) STRK1
Images
HA601001_1.jpg Fig1: Western blot analysis of PDE4D on Hela cell lysates with Mouse anti-PDE4D antibody (HA601001) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 91 kDa
Observed band size: 91 kDa

Exposure time: 30 seconds;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601001) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.