CD64 Mouse Monoclonal Antibody [A7E4]
cat.: HA601003
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A7E4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CD64 aa 1-200 / 374. |
| Positive control: | THP-1 cell lysates, U937 cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P12314 Human |
| Alternative names: | CD 64 CD64 CD64 antigen CD64A CD64b CD64c Fc fragment of IgG high affinity Ia receptor Fc fragment of IgG high affinity Ib receptor Fc fragment of IgG high affinity Ic receptor Fc fragment of IgG, high affinity Ia, receptor (CD64) Fc fragment of IgG, high affinity Ia, receptor for (CD64) Fc fragment of IgG, high affinity Ia, receptor for Fc fragment of IgG, high affinity Ib, receptor (CD64) Fc fragment of IgG, high affinity Ib, receptor for Fc fragment of IgG, high affinity Ic, receptor (CD64) Fc fragment of IgG, high affinity Ic, receptor (CD64), pseudogene Fc fragment of IgG, high affinity Ic, receptor for Fc gamma receptor Fc gamma receptor I A1 Fc gamma receptor I Fc gamma receptor I B2 Fc gamma RI Fc gamma RIA Fc gamma RIB Fc of IgG high affinity Ia receptor Fc-gamma RI Fc-gamma RIA Fc-gamma RIC FCG 1 FCG1 FcgammaRI FcgammaRIa FCGR 1 FCGR1 FCGR1_HUMAN FCGR1A FCGR1B FCGR1C FcRI FcRIB FCRIC FLJ18345 ...... |
Images
|
Fig1:
Western blot analysis of CD64 on THP-1 cell lysates with Mouse anti-CD64 antibody (HA601003) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 70 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601003) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CD64 on U937 cell lysates with Mouse anti-CD64 antibody (HA601003) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 70 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601003) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.