Caveolin-1 Mouse Monoclonal Antibody [A3E6]
cat.: HA601005
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: A3E6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 20 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human Caveolin-1 aa 1-150.
Positive control: SiHa cell lysates, human liver tissue, human lung tissue, human lung carcinoma tissue.
Subcellular location: Cell membrane, golgi apparatus membrane, trans-golgi network, caveola, membrane raft.
Recommended Dilutions:
  WB
  IHC-P

1:500
1:800-1:2,000
Uniprot #: SwissProt: Q03135 Human
Alternative names: BSCL3 CAV CAV1 CAV1_HUMAN caveolae protein, 22 kD caveolin 1 alpha isoform caveolin 1 beta isoform Caveolin 1 caveolae protein 22kDa Caveolin-1 Caveolin1 cell growth-inhibiting protein 32 CGL3 LCCNS MSTP085 OTTHUMP00000025031 PPH3 VIP 21 VIP21
Images
HA601005_1.jpg Fig1: Western blot analysis of Caveolin-1 on SiHa cell lysates with Mouse anti-Caveolin-1 antibody (HA601005) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 20 kDa
Observed band size: 20 kDa

Exposure time: 1 minute;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601005) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA601005_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Caveolin-1 antibody (HA601005) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601005) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601005_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue with Mouse anti-Caveolin-1 antibody (HA601005) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601005) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601005_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-Caveolin-1 antibody (HA601005) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601005) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.