STRAP Mouse Monoclonal Antibody [A7H11]
cat.: HA601007
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A7H11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human STRAP aa 201-350/350. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, A549 cell lysate, MCF-7 cell lysate, SH-SY5Y cell lysate, human testis tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:200 |
| Uniprot #: | SwissProt: Q9Y3F4 Human |
| Alternative names: | MAP activator with WD repeats MAWD PTWD Serine-threonine kinase receptor-associated protein serine/threonine kinase receptor associated protein strap STRAP_HUMAN UNR-interacting protein UNRIP WD 40 repeat protein PT WD WD-40 repeat protein PT-WD |
Images
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Fig1:
Western blot analysis of STRAP on different lysates with Mouse anti-STRAP antibody (HA601007) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601007) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of STRAP on different lysates with Mouse anti-STRAP antibody (HA601007) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601007) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-STRAP antibody (HA601007) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601007) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.