SIN1 Mouse Monoclonal Antibody [A6G10]
cat.: HA601008
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A6G10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human SIN1 aa 350-522. |
| Positive control: | Hela cell lysate, A549 cell lysate, Hela, human kidney tissue, MCF-7. |
| Subcellular location: | Nucleus, Cell membrane, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100 1:600 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9BPZ7 Human |
| Alternative names: | MAPKAP 1 MAPKAP1 MEKK2 interacting protein 1 MGC2745 MIP 1 MIP1 Mitogen activated protein kinase associated protein 1 Mitogen-activated protein kinase 2-associated protein 1 mSIN1 OTTHUMP00000064207 Ras inhibitor MGC2745 SAPK interacting protein 1 SAPK-interacting protein 1 SIN 1 SIN1_HUMAN SIN1b SIN1g Stress activated map kinase interacting protein 1 Stress activated protein kinase interacting 1 Stress-activated map kinase-interacting protein 1 Target of rapamycin complex 2 subunit MAPKAP1 TORC2 subunit MAPKAP1 |
Images
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Fig1:
Western blot analysis of SIN1 on different lysates with Mouse anti-SIN1 antibody (HA601008) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 60/70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601008) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling SIN1 with Mouse anti-SIN1 antibody (HA601008) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-SIN1 antibody (HA601008) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-SIN1 antibody (HA601008) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601008) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of MCF-7 cells labeling SIN1. Cells were fixed and permeabilized, and then blocked with 2% negative goat serum for 15 minutes at room temperature.Then stained with the primary antibody (HA601008, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.