SIN1 Mouse Monoclonal Antibody [A6G10]
cat.: HA601008
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A6G10
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 59 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human SIN1 aa 350-522.
Positive control: HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, U-87 MG cell lysate.
Subcellular location: Nucleus, Cell membrane, Cytoplasmic vesicle.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9BPZ7 Human
Alternative names: MAPKAP 1 MAPKAP1 MEKK2 interacting protein 1 MGC2745 MIP 1 MIP1 Mitogen activated protein kinase associated protein 1 Mitogen-activated protein kinase 2-associated protein 1 mSIN1 OTTHUMP00000064207 Ras inhibitor MGC2745 SAPK interacting protein 1 SAPK-interacting protein 1 SIN 1 SIN1_HUMAN SIN1b SIN1g Stress activated map kinase interacting protein 1 Stress activated protein kinase interacting 1 Stress-activated map kinase-interacting protein 1 Target of rapamycin complex 2 subunit MAPKAP1 TORC2 subunit MAPKAP1
Images
HA601008_1.jpg Fig1: Western blot analysis of SIN1 on different lysates with Mouse anti-SIN1 antibody (HA601008) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: U-87 MG cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 59 kDa
Observed band size: 65/75 kDa

Exposure time: 24 seconds; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601008) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.