| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A6G10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human SIN1 aa 350-522. |
| Positive control: | HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, U-87 MG cell lysate. |
| Subcellular location: | Nucleus, Cell membrane, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9BPZ7 Human |
| Alternative names: | MAPKAP 1 MAPKAP1 MEKK2 interacting protein 1 MGC2745 MIP 1 MIP1 Mitogen activated protein kinase associated protein 1 Mitogen-activated protein kinase 2-associated protein 1 mSIN1 OTTHUMP00000064207 Ras inhibitor MGC2745 SAPK interacting protein 1 SAPK-interacting protein 1 SIN 1 SIN1_HUMAN SIN1b SIN1g Stress activated map kinase interacting protein 1 Stress activated protein kinase interacting 1 Stress-activated map kinase-interacting protein 1 Target of rapamycin complex 2 subunit MAPKAP1 TORC2 subunit MAPKAP1 |
|
Fig1:
Western blot analysis of SIN1 on different lysates with Mouse anti-SIN1 antibody (HA601008) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: MCF7 cell lysate Lane 4: U-87 MG cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 65/75 kDa Exposure time: 24 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601008) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |