CD69 Mouse Monoclonal Antibody [A8C4]
cat.: HA601013
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A8C4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human CD69 aa 62-199/199. |
| Positive control: | Daudi cell lysate treated with PMA for 24 hours. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q07108 Human |
| Alternative names: | Activation inducer molecule (AIM/CD69) Activation inducer molecule AIM BL-AC/P26 BLAC/P26 C-type lectin domain family 2 member C CD69 CD69 antigen (p60, early T-cell activation antigen) CD69 antigen CD69 molecule CD69_HUMAN CLEC2C EA1 Early activation antigen CD69 Early lymphocyte activation antigen Early T cell activation antigen p60 Early T-cell activation antigen p60 GP32/28 Leu23 Leukocyte surface antigen Leu-23 MLR-3 MLR3 VEA Very Early Activation Antigen |
Images
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Fig1:
Western blot analysis of CD69 on different lysates with Mouse anti-CD69 antibody (HA601013) at 1/500 dilution. Lane 1: Daudi cell lysate Lane 2: Daudi cell lysate treated with PMA for 24 hours Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601013) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.