CD69 Mouse Monoclonal Antibody [A8C4]
cat.: HA601013
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A8C4
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within human CD69 aa 62-199/199.
Positive control: Daudi cell lysate treated with PMA for 24 hours.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q07108 Human
Alternative names: Activation inducer molecule (AIM/CD69) Activation inducer molecule AIM BL-AC/P26 BLAC/P26 C-type lectin domain family 2 member C CD69 CD69 antigen (p60, early T-cell activation antigen) CD69 antigen CD69 molecule CD69_HUMAN CLEC2C EA1 Early activation antigen CD69 Early lymphocyte activation antigen Early T cell activation antigen p60 Early T-cell activation antigen p60 GP32/28 Leu23 Leukocyte surface antigen Leu-23 MLR-3 MLR3 VEA Very Early Activation Antigen
Images
HA601013_1.jpg Fig1: Western blot analysis of CD69 on different lysates with Mouse anti-CD69 antibody (HA601013) at 1/1,000 dilution.

Lane 1: Daudi cell lysate
Lane 2: Daudi cell lysate treated with PMA for 24 hours

Lysates/proteins at 10 µg/Lane.
Exposure time: 90 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601013, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 23 kDa
Observed band size: 25 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.