VGluT1 Mouse Monoclonal Antibody [A7A3]
cat.: HA601014
| Product Type: | Mouse monoclonal IgG3, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A7A3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG3 |
| Immunogen: | Synthetic peptide within Human VGluT1 aa 501-550 / 560. |
| Positive control: | Rat brain tissue lysates, mouse brain tissue lysates. |
| Subcellular location: | Synaptic vesicle membrane, Membrane, Synaptosome. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q9P2U7 Human | Q3TXX4 Mouse | Q62634 Rat |
| Alternative names: | BNPI Brain specific Na (+) dependent inorganic phosphate cotransporter Brain specific Na dependent inorganic phosphate cotransporter Brain-specific Na(+)-dependent inorganic phosphate cotransporter Slc17a7 Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 Solute carrier family 17 member 7 Vesicular glutamate transporter 1 VGLU1_HUMAN VGLUT 1 VGluT1 |
Images
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Fig1:
Western blot analysis of VGluT1 on rat brain tissue lysates with Mouse anti-VGluT1 antibody (HA601014) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 53 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601014) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of VGluT1 on mouse brain tissue lysates with Mouse anti-VGluT1 antibody (HA601014) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 62 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601014) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.