IL-2 Mouse Monoclonal Antibody [A8B8]
cat.: HA601015
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, ELISA
Clonality: Monoclonal
Clone number: A8B8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 18 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human IL-2 aa 1-153/153.
Positive control: IL-2 recombinant protein.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  ELISA

1:500
1:20,000
Uniprot #: SwissProt: P60568 Human
Alternative names: Aldesleukin IL 2 IL-2 IL2 IL2_HUMAN Interleukin 2 Interleukin-2 interleukin2 Involved in regulation of T cell clonal expansion Lymphokine OTTHUMP00000164090 POIL2 T Cell Growth Factor T-cell growth factor TCGF
Images
HA601015_1.jpg Fig1: Western blot analysis of IL-2 on IL-2 recombinant protein with Mouse anti-IL-2 antibody (HA601015) at 1/500 dilution.

Lysates/proteins at 50 ng/Lane.

Exposure time: 30 seconds;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601015) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA601015_2.jpg Fig2: IL-2 Antibody (HA601015) in indirect ELISA.

Indirect ELISA analysis of IL-2 was performed by coating wells of a 96-well plate with 50 µl per well of IL-2 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse IL-2 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.