p21 Mouse Monoclonal Antibody [A8C11]
cat.: HA601018
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A8C11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within full length of human p21. |
| Positive control: | Human cervical well-differentiated squamous cell carcinoma tissue, human thyroid carcinoma tissue, human breast carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
IHC-P |
1:200-1:400 |
| Uniprot #: | SwissProt: P38936 Human |
| Alternative names: | CAP20 CDK-interacting protein 1 CDKI CDKN1 Cdkn1a CDN1A_HUMAN CIP1 Cyclin Dependent Kinase Inhibitor 1A Cyclin-dependent kinase inhibitor 1 Cyclin-dependent kinase inhibitor 1A (P21) Cyclin-dependent kinase inhibitor 1A (p21, Cip1) DNA Synthesis Inhibitor MDA-6 MDA6 Melanoma differentiation-associated protein 6 Melanoma differentiation-associated protein p21 P21 protein p21CIP1 p21Cip1/Waf1 p21WAF PIC1 SDI1 SLC12A9 WAF1 Wild type p53 activated fragment 1 (WAF1) Wild type p53 activated fragment 1 Wildtype p53-activated fragment 1 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human cervical well-differentiated squamous cell carcinoma tissue with Rabbit anti-p21 antibody (HA601018) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601018) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-p21 antibody (HA601018) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601018) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p21 antibody (HA601018) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601018) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.