BRG1 Mouse Monoclonal Antibody [A5C10]
cat.: HA601020
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A5C10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 185 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human BRG1 aa 240-290. |
| Positive control: | K562 cell lysates, HeLa, human kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:2,000 1:50- 1:200 1:100 |
| Uniprot #: | SwissProt: P51532 Human |
| Alternative names: | ATP dependent helicase SMARCA4 ATP-dependent helicase SMARCA4 BAF 190 BAF190 BAF190A Brahma protein like 1 BRG1 BRG1 associated factor 190A BRG1 protein BRG1-associated factor 190A BRM/SWI2 related gene 1 Global transcription activator homologous sequence global transcription activator snf2l4 Homeotic gene regulator hSNF2b Mitotic growth and transcription activator MRD16 Nuclear protein GRB1 Protein brahma homolog 1 Protein BRG-1 Protein BRG1 RTPS2 SMARC A4 SMARCA4 SMCA4_HUMAN SNF2 SNF2 beta SNF2 like 4 SNF2-beta SNF2B SNF2L4 SNF2LB Sucrose nonfermenting like 4 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 SWI2 Transcription activator BRG1 |
Images
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Fig1:
Western blot analysis of BRG1 on different lysates with Mouse anti-BRG1 antibody (HA601020) at 1/500 dilution. Lane 1: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 185 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 5% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601020) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling BRG1 with Mouse anti-BRG1 antibody (HA601020) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-BRG1 antibody (HA601020) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601020, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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