CD35 Mouse Monoclonal Antibody [A4F3]
cat.: HA601022
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC
Clonality: Monoclonal
Clone number: A4F3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 224 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within C-terminal human CD35.
Positive control: TF-1 cell lysates, human tonsil tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC

1:500
1:100-1:500
Uniprot #: SwissProt: P17927 Human
Alternative names: C3 binding protein C3b/C4b receptor C3BR C4BR CD 35 CD35 CD35 antigen complement component (3b/4b) receptor 1 (Knops blood group) complement component (3b/4b) receptor 1 including Knops blood group system Complement component receptor 1 Complement receptor 1 Complement receptor type 1 CR 1 CR1 CR1_HUMAN KN Knops blood group antigen
Images
HA601022_1.jpg Fig1: Western blot analysis of CD35 on TF-1 cell lysates with Mouse anti-CD35 antibody (HA601022) at 1/500 dilution.

Lane 1: TF-1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 224 kDa
Observed band size: 300 kDa

Exposure time: 2 minutes;

5% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601022) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
HA601022_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD35 antibody (HA601022) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601022) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.