HA601024

CD133 Mouse Monoclonal Antibody [A8C3]

cat.: HA601024

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human
Applications:	WB, IHC-P
Clonality:	Monoclonal
Clone number:	A8C3

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2ug/ul
Purification:	Protein G affinity purified.
Molecular weight:	Predicted band size: 97 kDa
Isotype:	IgG1
Immunogen:	Recombinant protein within human CD133 aa 151-400/865.
Positive control:	Caco-2 cell lysate, HT-29 cell lysate, NCCIT cell lysate, human kidney tissue lysates, human colon carcinoma tissue, human breast tissue, human kidney tissue.
Subcellular location:	Endoplasmic reticulum. Plasma membrane. Cell projection.
Recommended Dilutions: WB IHC-P	1:2,000 1:600
Uniprot #:	SwissProt: O43490 Human
Alternative names:	AC133 Antigen AC133 CD133 CORD12 Hematopoietic stem cell antigen hProminin MCDR2 MSTP061 OTTHUMP00000217744 OTTHUMP00000217745 OTTHUMP00000217746 PROM1 PROM1_HUMAN Prominin I Prominin like 1 Prominin like protein 1 precursor Prominin mouse like 1 Prominin-1 Prominin-like protein 1 Prominin1 PROML1 RP41 STGD4

Images

Fig1: Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601024) at 1/2,000 dilution.

Lane 1: Caco-2 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: NCCIT cell lysate
Lane 4: HeLa cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 97 kDa
Observed band size: 120 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601024) at 1/2,000 dilution.

Lane 1: HT-29 cell lysate
Lane 2: HT-29 cell lysate treated with deglycosylation

Lysates/proteins at 20 µg/Lane.

Predicted band size: 97 kDa
Observed band size: 120/97 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601024) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (10 µg/Lane) Lane 2: HT-29 cell lysate (10 µg/Lane) Lane 3: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601024) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (HA601024) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601024) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-CD133 antibody (HA601024) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601024) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD133 antibody (HA601024) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601024) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.