IL-28A Mouse Monoclonal Antibody [A5D6]
cat.: HA601027
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A5D6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide within human IL28A aa 110-160. |
| Positive control: | MCF7 cell lysate, HEK-293 cell lysate, SK-Br-3 cell lysate. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8IZJ0 Human |
| Alternative names: | Cytokine ZCYTO20 IFN-lambda-2 IFNL2 IL-28A IL28A IL28A_HUMAN Interferon lambda 2 Interferon lambda-2 interleukin 28A (interferon, lambda 2) Interleukin 28A Interleukin-28A ZCYTO20 |
Images
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Fig1:
Western blot analysis of IL-28A on different lysates with Mouse anti-IL-28A antibody (HA601027) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: SK-Br-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.