IL-28A Mouse Monoclonal Antibody [A5D6]
cat.: HA601027
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A5D6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG2a
Immunogen: Synthetic peptide within human IL28A aa 110-160.
Positive control: MCF7 cell lysate, HEK-293 cell lysate, SK-Br-3 cell lysate.
Subcellular location: Secreted
Recommended Dilutions:
  WB

1:500-1:1,000
Uniprot #: SwissProt: Q8IZJ0 Human
Alternative names: Cytokine ZCYTO20 IFN-lambda-2 IFNL2 IL-28A IL28A IL28A_HUMAN Interferon lambda 2 Interferon lambda-2 interleukin 28A (interferon, lambda 2) Interleukin 28A Interleukin-28A ZCYTO20
Images
HA601027_1.jpg Fig1: Western blot analysis of IL-28A on different lysates with Mouse anti-IL-28A antibody (HA601027) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: SK-Br-3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.