Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12]
cat.: HA601028
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A3F12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 174/178/182 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Topoisomerase II alpha aa1480-1531. |
| Positive control: | A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human breast carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200 |
| Uniprot #: | SwissProt: P11388 Human | Q01320 Mouse | P41516 Rat |
| Alternative names: | alpha isozyme ATP hydrolyzing DNA topoisomerase II alfa DNA gyrase DNA topoisomerase (ATP hydrolyzing) DNA topoisomerase 2 alpha DNA topoisomerase 2-alpha DNA topoisomerase II 170 kD DNA topoisomerase II alpha isozyme DNA topoisomerase II DNA Topoisomerase2 TOP 2A TOP2 TOP2A TOP2A_HUMAN Topoisomerase DNA II alpha 170kDa TP2A |
Images
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Fig1:
Western blot analysis of Topoisomerase II alpha on different cell lysates with Mouse anti-Topoisomerase II alpha antibody (HA601028) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 174 kDa Observed band size: 174 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601028) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Topoisomerase II alpha antibody (HA601028) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601028) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.