Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | A7C12 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | Predicted band size: 52 kDa |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide within human MUM1 aa 402-451/451. |
Positive control: | Human colon tissue, human lymph nodes tissue. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
IHC-P |
1:2,000 |
Uniprot #: | SwissProt: Q15306 Human |
Alternative names: | Interferon regulatory factor 4 IRF 4 IRF-4 Irf4 IRF4_HUMAN LSIRF Lymphocyte specific interferon regulatory factor Lymphocyte specific IRF Lymphocyte-specific interferon regulatory factor Multiple myeloma oncogene 1 MUM 1 MUM1 NF EM5 NF-EM5 NFEM5 PU.1 interaction partner Sfpi1/PU.1 interaction partner Transcriptional activator PIP |
Fig1:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-MUM1 antibody (HA601031) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601031) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-MUM1 antibody (HA601031) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601031) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |