GM-CSF Mouse Monoclonal Antibody [A6C5]
cat.: HA601033
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | A6C5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human GM-CSF aa 1-144/144. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB ELISA |
1:2,000 1:30,000 |
| Uniprot #: | SwissProt: P04141 Human |
| Alternative names: | Colony stimulating factor 2 (granulocyte-macrophage) Colony Stimulating Factor 2 Colony stimulating factor Colony-stimulating factor CSF 2 CSF CSF2 CSF2_HUMAN GM-CSF GMCSF Granulocyte Macrophage Colony Stimulating Factor Granulocyte-macrophage colony-stimulating factor MGC131935 MGC138897 MGI1GM Molgramostin Pluripoietin-a Sargramostim |
Images
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Fig1:
Western blot analysis of GM-CSF on recombinant protein with Mouse anti-GM-CSF antibody (HA601033) at 1/2,000 dilution. Lysates/proteins at 50 ng/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601033) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
GM-CSF Antibody (HA601033) in indirect ELISA. Indirect ELISA analysis of GM-CSF was performed by coating wells of a 96-well plate with 50 µl per well of GM-CSF antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse GM-CSF monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.