HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]
cat.: HA601037
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A2-F6-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within N-terminal human Beta-actin . |
| Positive control: | HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, L6 cell lysate, MEF cell lysate, HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,500-1:20,000 |
| Uniprot #: | SwissProt: P60709 Human | P60710 Mouse | P60711 Rat |
| Alternative names: | A26C1A A26C1B ACTB ACTB_HUMAN Actin beta Actin cytoplasmic 1 Actin, cytoplasmic 1, N-terminally processed Actx b actin Beta cytoskeletal actin Beta-actin BRWS1 E430023M04Rik MGC128179 PS1TP5 binding protein 1 PS1TP5BP1 β-actin β actin |
Images
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Fig1:
Western blot analysis of beta Actin on HepG2 cell lysates with Rabbit anti-beta Actin antibody (HA601037) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig2:
Western blot analysis of beta Actin on NIH/3T3 cell lysates with Rabbit anti-beta Actin antibody (HA601037) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig3:
Western blot analysis of beta Actin on PC-12 cell lysates with Rabbit anti-beta Actin antibody (HA601037) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig4:
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601037) at 1/10,000 dilution. Lane 1: THP-1 cell lysate Lane 2: MCF-7 cell lysate Lane 3: RH-35 cell lysate Lane 4: L6 cell lysate Lane 5: MEF cell lysate Lane 6: HepG2 cell lysate Lane 7: PC-12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: RAW264.7 cell lysate Lane 10: A549 cell lysate Lane 11: PMVEC cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.