uPA Receptor Mouse Monoclonal Antibody [A6E8]
cat.: HA601039
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A6E8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human uPA Receptor aa 51-250/335.
Positive control: U937 treated with 200 nM PMA for 72 hours cell lysate.
Subcellular location: Cell membrane, invadopodium membrane; Secreted.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q03405 Human
Alternative names: CD 87 CD87 CD87 antigen MO 3 MO3 Monocyte activation antigen Mo3 Plasminogen activator receptor urokinase Plasminogen activator urokinase receptor PLAUR U PAR u plasminogen activator receptor U-PAR u-plasminogen activator receptor form 2 UPA receptor uPAR UPAR_HUMAN Urinary plasminogen activator receptor URKR Urokinase plasminogen activator receptor Urokinase plasminogen activator surface receptor urokinase-type plasminogen activator (uPA) receptor
Images
HA601039_1.jpg Fig1: Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody (HA601039) at 1/1,000 dilution.

Lane 1: U937 cell lysate
Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 37 kDa
Observed band size: 37-60 kDa

Exposure time: 15 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.