TBK1 Mouse Monoclonal Antibody [A8D10]
cat.: HA601044
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A8D10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human TBK1 aa 530-729/729. |
| Positive control: | K562 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat stomach tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q9UHD2 Human | Q9WUN2 Mouse | D4A7D3 Rat |
| Alternative names: | EC 2.7.11.1 FLJ11330 FTDALS4 NAK NF kappa B activating kinase NF kB activating kinase NF-kappa-B-activating kinase Serine/threonine protein kinase TBK 1 Serine/threonine protein kinase TBK1 Serine/threonine-protein kinase TBK1 T2K TANK binding kinase 1 TANK-binding kinase 1 TBK 1 Tbk1 TBK1_HUMAN |
Images
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Fig1:
Western blot analysis of TBK1 on different lysates with Mouse anti-TBK1 antibody (HA601044) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: Hela cell lysate Lane 4: MCF-7 cell lysate Lane 5: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TBK1 on different lysates with Mouse anti-TBK1 antibody (HA601044) at 1/500 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Rat stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of TBK1 on different lysates with Mouse anti-TBK1 antibody (HA601044) at 1/500 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si TBK1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.