NAK / TBK1 Mouse Monoclonal Antibody [A8D10]
cat.: HA601044
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A8D10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human TBK1 aa 530-729/729. |
| Positive control: | HCT 116 cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7 cell lysate, A-172 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q9UHD2 Human | Q9WUN2 Mouse | D4A7D3 Rat |
| Alternative names: | EC 2.7.11.1 FLJ11330 FTDALS4 NAK NF kappa B activating kinase NF kB activating kinase NF-kappa-B-activating kinase Serine/threonine protein kinase TBK 1 Serine/threonine protein kinase TBK1 Serine/threonine-protein kinase TBK1 T2K TANK binding kinase 1 TANK-binding kinase 1 TBK 1 Tbk1 TBK1_HUMAN |
Images
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Fig1:
Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: A-172 cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: Mouse testis tissue lysate (40 µg/Lane) Lane 9: Rat testis tissue lysate (40 µg/Lane) Lane 10: Mouse lung tissue lysate (40 µg/Lane) Lane 11: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/500 dilution. Lane 1: A549-si NT cell lysate (20 µg/Lane) Lane 2: A549-si NAK / TBK1 cell lysate (20 µg/Lane) Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.