GSDMD Mouse Monoclonal Antibody [A8D7]
cat.: HA601046
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A8D7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human GSDMD aa 101-300/484. |
| Positive control: | PC-3 cell lysate, NIH/3T3 cell lysate, THP-1 cell lysate. |
| Subcellular location: | Cytosol, Inflammasome; Secreted, Cell membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P57764 Human | Q9D8T2 Mouse |
| Alternative names: | 1810036L03Rik DF 5L DF5L DFNA 5L DFNA5L FKSG 10 FKSG10 FLJ12150 Gasdermin Gasdermin D Gasdermin domain containing 1 Gasdermin domain containing protein 1 Gasdermin domain-containing protein 1 Gasdermin-D GasderminD GSDMD GSDMD_HUMAN GSDMDC 1 GSDMDC1 |
Images
|
Fig1:
Western blot analysis of GSDMD on different lysates with Mouse anti-GSDMD antibody (HA601046) at 1/500 dilution. Lane 1: PC-3 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601046) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.