IL-7 Mouse Monoclonal Antibody [A8B11]
cat.: HA601048
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A8B11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human IL-7 aa 1-177/177. |
| Positive control: | HepG2 cell lysates. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P13232 Human |
| Alternative names: | IL 7 IL-7 Il7 IL7_HUMAN Interleukin 7 Interleukin-7 LP-1 Lymphopoietin 1 |
Images
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Fig1:
Western blot analysis of IL-7 on HepG2 cell lysates with Mouse anti-IL-7 antibody (HA601048) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601048) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.