Lambda Light Chain Mouse Monoclonal Antibody [A8G5]
cat.: HA601055
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A8G5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG2b |
| Immunogen: | Constant region of natural protein (constant region of light chain lambda chain). |
| Positive control: | Human small intestine tissue lysates, human plasma lysates, human tonsil tissue, Ramos. |
| Subcellular location: | Secreted, Cell membrane. |
| Recommended Dilutions:
IHC-P WB IF-Cell FC |
1:4,000 1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P0CG04 Human |
| Alternative names: | Constant region of lambda light chains Ig lambda chain C regions IGLC Immunoglobulin lambda constant 1 Mcg marker |
Images
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Fig1:
Western blot analysis of Lambda Light Chain on human small intestine tissue lysates with Mouse anti-Lambda Light Chain antibody (HA601055) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 25 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601055) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Lambda Light Chain on human plasma lysates with Mouse anti-Lambda Light Chain antibody (HA601055) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 11 kDa Observed band size: 25 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601055) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Lambda Light Chain antibody (HA601055) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601055) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Ramos cells labeling Lambda Light Chain with Mouse anti-Lambda Light Chain antibody (HA601055) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lambda Light Chain antibody (HA601055) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of Ramos cells labeling Lambda Light Chain. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601055, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.