HRP Conjugated M13 Recombinant Mouse Monoclonal Antibody [PS00-01]
cat.: HA601057
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | M13 phage |
| Applications: | ELISA |
| Clonality: | Monoclonal |
| Clone number: | PS00-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | M13 Bacteriophage. |
| Positive control: | M13 Bacteriophage. |
| Recommended Dilutions:
ELISA |
1:4,000-1:30,000 |
| Uniprot #: | SwissProt: P69541 BacteriophageM13 |
| Alternative names: | Bacteriophage M13 Coliphage M13 Enterobacteria phage M13 M13 virus Phage M13 |
Images
|
Fig1: Direct ELISA analysis of M13 was performed by coating HSA/BSA (2 μg/mL in pH 9.6 carbonate buffer) in 96-well plate, and then add the indicated amounts of M13 Bacteriophages displaying anti-HSA Fab overnight at 4℃. Incubated with 100 µl per well of anti-M13 monoclonal antibody starting at a dilution of 1/1,000 and serially diluting it to a dilution of 1:64,000 for 1 hours at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.