KRAS Mouse Monoclonal Antibody [A8E4]
cat.: HA601058
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A8E4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human KRAS aa 2-186. |
| Positive control: | Rat brain tissue lysate, Jurkat cell lysate, N2A cell lysate, PC-12 cell lysate, human kidney tissue lysate, Hela, NIH/3T3. |
| Subcellular location: | Cell membrane. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P01116 Human | P32883 Mouse | P08644 Rat |
| Alternative names: | c Ki ras2 c Kirsten ras protein c-K-ras c-Ki-ras Cellular c Ki ras2 proto oncogene Cellular transforming proto oncogene CFC2 cK Ras GTPase KRas K RAS p21 protein K RAS2A K RAS2B K RAS4A K RAS4B K-Ras 2 KI RAS Ki-Ras KIRSTEN MURINE SARCOMA VIRUS 2 Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog Kirsten rat sarcoma viral oncogene homolog KRAS KRAS proto oncogene, GTPase KRAS1 KRAS2 N-terminally processed NS NS3 Oncogene KRAS2 p21ras PR310 c K ras oncogene PR310 cK ras oncogene RALD RASK_HUMAN RASK2 Transforming protein p21 v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog v Ki ras2 Kirsten rat sarcoma viral oncogene homolog |
Images
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Fig1:
Western blot analysis of KRAS on different lysates with Mouse anti-KRAS antibody (HA601058) at 1/500 dilution. Lane 1: Rat brain tissue lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: N2A cell lysate (10 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601058) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of KRAS on different lysates with Mouse anti-KRAS antibody (HA601058) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 µg/Lane) Lane 2: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601058) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of Hela cells labeling KRAS with Mouse anti-KRAS antibody (HA601058) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-KRAS antibody (HA601058) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Flow cytometric analysis of NIH/3T3 cells labeling KRAS. Cells were fixed and permeabilized, and then blocked with 2% negative goat serum for 15 minutes at room temperature.Then stained with the primary antibody (HA601058, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.