KRAS Mouse Monoclonal Antibody [A8E6]
cat.: HA601060
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A8E6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human KRAS aa 2-186. |
| Positive control: | HEK-293 cell lysate, 22RV1 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat brain tissue lysate, mouse colon tissue, mouse kidney tissue, rat colon tissue. |
| Subcellular location: | Cell membrane. Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P01116 Human | P32883 Mouse | P08644 Rat |
| Alternative names: | c Ki ras2 c Kirsten ras protein c-K-ras c-Ki-ras Cellular c Ki ras2 proto oncogene Cellular transforming proto oncogene CFC2 cK Ras GTPase KRas K RAS p21 protein K RAS2A K RAS2B K RAS4A K RAS4B K-Ras 2 KI RAS Ki-Ras KIRSTEN MURINE SARCOMA VIRUS 2 Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog Kirsten rat sarcoma viral oncogene homolog KRAS KRAS proto oncogene, GTPase KRAS1 KRAS2 N-terminally processed NS NS3 Oncogene KRAS2 p21ras PR310 c K ras oncogene PR310 cK ras oncogene RALD RASK_HUMAN RASK2 Transforming protein p21 v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog v Ki ras2 Kirsten rat sarcoma viral oncogene homolog |
Images
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Fig1:
Western blot analysis of KRAS on different lysates with Mouse anti-KRAS antibody (HA601060) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: 22RV1 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse kidney tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601060) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-KRAS antibody (HA601060) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601060) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-KRAS antibody (HA601060) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601060) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-KRAS antibody (HA601060) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601060) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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