CD161 Mouse Monoclonal Antibody [A8E1]
cat.: HA601063
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A8E1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within human CD161 aa 176-225. |
| Positive control: | THP-1 cell lysate, HL-60 cell lysate, K-562 cell lysate, Jurkat cell lysate, human kidney tissue lysate, human kidney tissue, THP-1. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q12918 Human |
| Alternative names: | C-type lectin domain family 5 member B CD161 CLEC5B HNKR-P1a Killer Cell Lectin like Receptor Subfamily B Member 1 Killer cell lectin-like receptor subfamily B member 1 KLRB1 KLRB1_HUMAN Natural killer cell surface protein P1A NKR NKR P1 NKR-P1A NKRP1 NKRP1A |
Images
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Fig1:
Western blot analysis of CD161 on different lysates with Mouse anti-CD161 antibody (HA601063) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: HL-60 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Human kidney tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601063) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD161 antibody (HA601063) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601063) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of THP-1 cells labeling CD161. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601063, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.