TCP1 delta Mouse Monoclonal Antibody [A8E2]
cat.: HA601064
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A8E2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human TCP1 delta aa 200-450. |
| Positive control: | Hela cell lysate, A549 cell lysate, PC-12 cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, PC-3M cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, human breast carcinoma tissue, human stomach tissue. |
| Subcellular location: | Cytoskeleton |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:800 |
| Uniprot #: | SwissProt: P50991 Human | P80315 Mouse | Q7TPB1 Rat |
| Alternative names: | CCT delta CCT4 CCTD chaperonin containing t-complex polypeptide 1, delta subunit chaperonin containing TCP1, subunit 4 (delta) SRB Stimulator of TAR RNA binding T complex protein 1 subunit delta TCP-1-delta |
Images
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Fig1:
Western blot analysis of TCP1 delta on different lysates with Mouse anti-TCP1 delta antibody (HA601064) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: PC-12 cell lysate (10 µg/Lane) Lane 4: SH-SY5Y cell lysate (10 µg/Lane) Lane 5: THP-1 cell lysate (10 µg/Lane) Lane 6: PC-3M cell lysate (10 µg/Lane) Lane 7: Jurkat cell lysate (10 µg/Lane) Lane 8: Mouse testis tissue lysate (20 µg/Lane) Lane 9: Rat spleen tissue lysate (20 µg/Lane) Lane 10: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601064) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-TCP1 delta antibody (HA601064) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601064) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-TCP1 delta antibody (HA601064) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601064) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.