ACSL3 Mouse Monoclonal Antibody [A8F6]
cat.: HA601069
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A8F6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1.18ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 80 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human ACSL3 aa 180-380.
Positive control: HeLa cell lysate, K-562 cell lysate, A431 cell lysate, 293T cell lysate.
Subcellular location: Mitochondrion, Peroxisome, Endoplasmic reticulum.
Recommended Dilutions:
  WB
1:1,000-1:5,000
Uniprot #: SwissProt: O95573 Human
Alternative names: ACS3 Acsl3 ACSL3_HUMAN Acyl CoA synthetase long chain family member 3 FACL3 Fatty acid Coenzyme A ligase long chain 3 LACS 3 LACS3 Lignoceroyl CoA synthase Long chain acyl CoA synthetase 3 Long chain fatty acid CoA ligase 3 Long-chain acyl-CoA synthetase 3 Long-chain-fatty-acid--CoA ligase 3 PRO2194
Images
HA601069_1.jpg Fig1: Western blot analysis of ACSL3 on different lysates with Mouse anti-ACSL3 antibody (HA601069) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: A431 cell lysate
Lane 4: 293T cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 80 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601069) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.