ACSL3 Mouse Monoclonal Antibody [A8F7]
cat.: HA601070
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A8F7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.16ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human ACSL3 aa 180-380. |
| Positive control: | K562 cell lysates, Hela cell lysates, 293T cell lysates, human liver tissue. |
| Subcellular location: | Mitochondrion, Peroxisome, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:240 |
| Uniprot #: | SwissProt: O95573 Human | Q9CZW4 Mouse | Q63151 Rat |
| Alternative names: | ACS3 Acsl3 ACSL3_HUMAN Acyl CoA synthetase long chain family member 3 FACL3 Fatty acid Coenzyme A ligase long chain 3 LACS 3 LACS3 Lignoceroyl CoA synthase Long chain acyl CoA synthetase 3 Long chain fatty acid CoA ligase 3 Long-chain acyl-CoA synthetase 3 Long-chain-fatty-acid--CoA ligase 3 PRO2194 |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL3 antibody (HA601070) at 1/240 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601070) at 1/240 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of ACSL3 on different lysates with Rabbit anti-ACSL3 antibody (HA601070) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 3: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 4: 293T (Human embryonic kidney cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minuties ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601070, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 80 kDa Observed band size: 75 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.