ACSL3 Mouse Monoclonal Antibody [A8F7]
cat.: HA601070
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A8F7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.16ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human ACSL3 aa 180-380. |
| Positive control: | K562 cell lysates, Hela cell lysates, 293T cell lysates, human liver tissue. |
| Subcellular location: | Mitochondrion, Peroxisome, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:240 |
| Uniprot #: | SwissProt: O95573 Human | Q9CZW4 Mouse | Q63151 Rat |
| Alternative names: | ACS3 Acsl3 ACSL3_HUMAN Acyl CoA synthetase long chain family member 3 FACL3 Fatty acid Coenzyme A ligase long chain 3 LACS 3 LACS3 Lignoceroyl CoA synthase Long chain acyl CoA synthetase 3 Long chain fatty acid CoA ligase 3 Long-chain acyl-CoA synthetase 3 Long-chain-fatty-acid--CoA ligase 3 PRO2194 |
Images
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Fig1:
Western blot analysis of ACSL3 on K562 cell lysates with Mouse anti-ACSL3 antibody (HA601070) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 70 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601070) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ACSL3 on Hela cell lysates with Mouse anti-ACSL3 antibody (HA601070) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601070) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of ACSL3 on 293T cell lysates with Mouse anti-ACSL3 antibody (HA601070) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601070) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL3 antibody (HA601070) at 1/240 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601070) at 1/240 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.