xCT / SLC7A11 Recombinant Mouse Monoclonal Antibody [A7C6-R]
cat.: HA601071
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A7C6-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG1 |
| Immunogen: | 293 cell line overexpressing SLC7A11. |
| Positive control: | HCT 116 (Human colon cancer cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, SW480 (Human colorectal zdenocarcinoma cell) cell lysate, COLO205 (Human colon adenocarcinoma cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, human pancreas tissue, HT-29. |
| Subcellular location: | Cell membrane, Cell projection, microvillus membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:10,000 1:200 1:100-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q9UPY5 Human | Q9WTR6 Mouse |
| Alternative names: | Amino acid transport system xc xCT Amino acid transport system xc- Calcium channel blocker resistance protein CCBR1 Calcium channel blocker resistance protein CCBR1 CCBR1 Cysteine/glutamate transporter Cystine/glutamate transporter OTTHUMP00000164578 SLC7A11 Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 solute carrier family 7 Solute carrier family 7 member 11 Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN xCT XCT_HUMAN |
Images
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Fig1:
Western blot analysis of xCT / SLC7A11 on different lysates with Rabbit anti-xCT / SLC7A11 antibody (HA601071) at 1/10,000 dilution. Lane 1: HCT 116 (Human colon cancer cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 3: SW480 (Human colorectal zdenocarcinoma cell) cell lysate Lane 4: COLO205 (Human colon adenocarcinoma cell) cell lysate Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Low expression: COLO205 (PMID: 31000598). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601071, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 55 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT / SLC7A11 antibody (HA601071) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601071) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of HT-29 cells labeling xCT / SLC7A11 with Mouse anti-xCT / SLC7A11 antibody (HA601071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-xCT / SLC7A11 antibody (HA601071) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of HT-29 cells labeling xCT / SLC7A11. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601071, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.