GNAS Mouse Monoclonal Antibody [A8F12]
cat.: HA601074
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A8F12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.19ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within human GNAS aa 2-251. |
| Positive control: | HepG2 cell lysate, NIH/3T3 cell lysate, human brain tissue lysate, rat pancreas tissue, human pancreas tissue, MCF-7, human breast carcinoma tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200-1:500 1:200 |
| Uniprot #: | SwissProt: P63092 Human | P84996 Human | Q5JWF2 Human | P63094 Mouse | Q6R0H7 Mouse | P63095 Rat | Q63803 Rat |
| Alternative names: | Adenylate cyclase stimulating G alpha protein AHO Alternative gene product encoded by XL exon C20orf45 dJ309F20.1.1 dJ806M20.3.3 Extra large alphas protein GNAS GNAS complex locus GNAS1 GPSA Gs alpha subunit GSA GSP Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1 Guanine nucleotide binding protein alpha stimulating activity polypeptide 1 Guanine nucleotide binding protein G(s) subunit alpha isoforms short Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas Guanine nucleotide regulatory protein MGC33735 NESP NESP55 Neuroendocrine secretory protein PHP1A PHP1B PHP1C POH Protein ALEX Protein GNAS Protein NESP55 SCG6 Secretogranin VI XLalphas XLas |
Images
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Fig1:
Western blot analysis of GNAS on different lysates with Mouse anti-GNAS antibody (HA601074) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Human brain tissue lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601074) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-GNAS antibody (HA601074) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601074) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-GNAS antibody (HA601074) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601074) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of MCF-7 cells labeling GNAS with Mouse anti-GNAS antibody (HA601074) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GNAS antibody (HA601074) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-GNAS antibody (HA601074) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601074) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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