6X His tag Recombinant Mouse Monoclonal Antibody [A5D1-R]
cat.: HA601079
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | A5D1-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide HHHHHHGCGHHHHHH. |
| Positive control: | C-terminal His-tag fusion protein lysates, Mid His-tag fusion protein lysates. |
| Recommended Dilutions:
WB WB IP |
1:5,000-1:50,000 (C-His) 1:1,000-1:5,000 (Mid-His) Use at an assay dependent concentration (C-His) |
| Alternative names: | 6 His epitope tag Hexa His tag HHHHHH epitope tag HHHHHH tag His tag Polyhistidine Tag |
Images
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Fig1:
Western blot analysis of 6X His tag on different lysates with Mouse anti-6X His tag antibody (HA601079) at different dilutions. Lane 1/2: C-terminal His-tag fusion protein lysates Lane 3/4: Mid His-tag fusion protein lysates Lysates/proteins at 50 ng/Lane. Observed band size: 30/40 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601079) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
His-tag was immunoprecipitated in 2µg C-terminal His-tag fusion protein lysate with HA601079. Western blot was performed from the immunoprecipitate using HA601079 at 1/2,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 60 mins at room temperature. Lane 1: C-terminal His-tag fusion protein lysate (input). Lane 2: HA601079 IP in C-terminal His-tag fusion protein lysate. Lane 3: Mouse IgG instead of HA601079 IP in C-terminal His-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.