beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]
cat.: HA601082
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A2-F6-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within N-terminal human Beta-actin . |
| Positive control: | HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, MCF-7 cell lysate, THP-1 cell lysate, PMVEC cell lysate, RH-35 cell lysate, RAW264.7 cell lysate, B16F1 cell lysate, MEF cell lysate, Hela cell lysate, F9 cell lysate, Mouse lung cell lysate, L6 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000-1:80,000 |
| Uniprot #: | SwissProt: P60709 Human | P60710 Mouse | P60711 Rat |
| Alternative names: | A26C1A A26C1B ACTB ACTB_HUMAN Actin beta Actin cytoplasmic 1 Actin, cytoplasmic 1, N-terminally processed Actx b actin Beta cytoskeletal actin Beta-actin BRWS1 E430023M04Rik MGC128179 PS1TP5 binding protein 1 PS1TP5BP1 β-actin β actin |
Images
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Fig1:
Western blot analysis of beta Actin on HepG2 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of beta Actin on NIH/3T3 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of beta Actin on PC-12 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601082) at 1/20,000 dilution. Lane 1: Hela (Human cervical adenocarcinoma cell) cell lysate Lane 2: HepG2 (Human hepatocellular carcinoma cell) cell lysate Lane 3: MCF-7 (Human breast cancer cell) cell lysate Lane 4: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 5: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 6: HEK-293 (Human embryonic kidney cell) cell lysate Lane 7: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 8: C2C12 (Mouse myoblast) cell lysate Lane 9: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 10: mouse testis tissue lysate Lane 11: mouse kidney tissue lysate Lane 12: mouse testis tissue lysate Lane 13: mouse kidney tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601082, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42 kDa Observed band size: 42 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.